Skip to:

Login

Biological Nanostructures Laboratory - Genetics Core

The Biological NanoStructures Lab (BNL) is a 1600 square foot shared multi-user environment for biological synthesis and analysis. Instrumentation for molecular biology, recombinant protein expression, purification & analysis and tissue culture will be available on a recharge basis.

This core provides Next Generation Sequencing (NGS) library prep and sequencing resources to on- and off-campus researchers near Santa Barbara, CA. NGS (aka High Throughput Sequencing) experiments are conducted on the Illumina NextSeq 500 which uses the same widely accepted SBS technology found in other popular Illumina sequencers such as the HiSeq and MiSeq.

Access available to the following: 
Collaborators
Undergrads
CampusAll
Academic
Government
Industry
International
Access Procedure: 

Trained users will have keycard access to the lab and will be able to book and use the analytical instruments. For ease of management, molecular biology instruments located in the BNL are also part of the Analytical core. Researchers can also engage BNL staff to perform basic molecular biological experiments such as recombinant protein expression. Cost will be on a project-by-project basis and will include staff man-hours, materials used and instrument recharges.

Steps to Use Equipment
  1. Contact BNL staff to get trained on the instrument.
  2. Apply to CNSI for electronic door access to the facility.
  3. BNL Staff will add you to the FBS reservation system.

 

Instruments

  1. Agilent TapeStation »
    Campus Gov't/Academic Industry

    The TapeStation is used for nucleic acid analysis. Genomic DNA, RNA and prepped dsDNA libraries can be analyzed with the instrument to get size distribution and concentration estimates.

  2. Bio Rad QX200 Droplet Digital PCR »
    Campus Gov't/Academic Industry

    Precise and sensitive digital PCR for a wide variety of applications, flexible PCR chemistry with user-friendly software for experimental set up and analysis.

  3. Covaris M220 Focused-Ultrasonicator »
    Campus Gov't/Academic Industry

    The Covaris M220 is designed for Next-Gen Sequencing applications requiring fragment sizes between 150bp and 5kb.

  4. Illumina NextSeq 500 »
    Campus Gov't/Academic Industry

    The NextSeq 500 desktop sequencing system provides the flexible power and simplicity to analyze whole genomes, exomes, and transcriptomes.

  5. Nuaire Biological Safety Hood »
    Campus Gov't/Academic Industry

    Class II type A2 Biosafety Cabinet.

  6. Qubit 2.0 Fluorometer »
    Campus Gov't/Academic Industry

    The Qubit 2.0 Fluorometer is a benchtop fluorometer for the quantitation of DNA, RNA, and protein, using the highly sensitive and accurate fluorescence-based Qubit quantitation assays.

  7. Sage Science Pippin Prep »
    Campus Gov't/Academic Industry

    The Pippin Prep allows researchers to easily collect, from mixtures of DNA fragments, narrow or wide size distributions with minimal effort.