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Gel Permeation Chromatography (GPC) - Chloroform

Gel Permeation Chromatography (GPC) is a separation method for the determination of molecular weight averages (Mn) and molecular weight distribution (PDI=Mw/Mn). The testing report can include calibration plot, chromatogram, number average molecular weight and PDI of the polymer. GPC-chloroform is using chloroform as a solvent. Gel Permeation Chromatography (GPC) separates molecules on the basis of hydrodynamic size (not Mw). The large molecules move more rapidly through the GPC column, and in this way the mixture can be separated. The elution volume of a molecule is related to its hydrodynamic volume. To relate the elution volume to the molecular weight of a polymer, a calibration with molecular weight standards is needed.

Technical Primary Contact:
Rachel Behrens

Access Procedure: 
Users of the MRL Synthesis and Characterization Laboratory are required to go through two levels of training before they are authorized to perform data collection independently. The training consists of
  • General lab safety training, provided by the UCSB Environmental Health & Safety Department (EH&S).
  • Instrument training, conducted by development engineer or an authorized user delegated by the development engineer.