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Biological Nanostructures Laboratory - Analytical Core

The Biological NanoStructures Lab (BNL) is a 1600 square foot shared multi-user environment for biological synthesis and analysis. Instrumentation for molecular biology, recombinant protein expression, purification & analysis and tissue culture will be available on a recharge basis.

The Analytical core of BNL houses a variety of analytical instruments for use by trained users. Some basic molecular biology equipment are also available for use.

Access available to the following: 
Collaborators
Undergrads
CampusAll
Academic
Industry
Access Procedure: 
Trained users will have keycard access to the lab and will be able to book and use the analytical instruments. For ease of management, molecular biology instruments located in the BNL are also part of the Analytical core. Researchers can also engage BNL staff to perform basic molecular biological experiments such as recombinant protein expression. Cost will be on a project-by-project basis and will include staff man-hours, materials used and instrument recharges.
 
Steps to Use Equipment
  1. Contact BNL staff to get trained on the instrument.
  2. Apply to CNSI for electronic door access to the facility.
  3. BNL Staff will add you to the FBS reservation system.

 

Instruments

  1. Beckman-Coulter Optima XE-90 ultracentrifuge

    The Beckman-Coulter Optima XE-90 ultracentrifuge offers fast, user-friendly centrifugation at speeds up to 90,000 RPM

  2. Conductivity / pH Meter

    Fisherbrand accumet AB200 pH/conductivity meter combines the benefits of a pH meter and conductivity meter in one easy to use, reliable measurement system.

  3. Malvern NanoSight NS300

    Nanoparticle characterization tool that precisely measures size distribution and concentration of particles suspended in solution using nanoparticle tracking analysis.

  4. Malvern Zetasizer Nano ZS

    The Malvern Zetasizer Nano system is a dynamic light scattering (DLS) machine capable of measuring particle size and zeta potential.

  5. Milli-Q Advantage A10 Water System

    Milli-Q water at a resistivity of 18.2 mΩ.cm and 3 ppb.

  6. MiSonix Ultra Sonicator

    Digital sonicating probe, 200 ul minimum volume for ultrasonic liquid processing.

  7. New Brunswick Innova 44R Shakers

    Two large volume temperature-controlled orbital shakers with adjustable speeds.

  8. Other Analytical Core Instruments

    Additional measurement, molecular biology and biochemistry tools available for use with daily recharge.

  9. Sartorius Octet R4

    The Sartorius Octet R4 system uses Bio-Layer Interferometry (BLI) for label-free, fast, and sensitive protein-binding kinetics and quantitation. The Octet software suite offers user-friendly assay set up and analysis.

  10. Shimadzu UV-1800 UV-Vis Spectrophotometer

    The UV-1800 has a wavelength range of 190 - 1100 nm with exceptional precision.

  11. Sorvall Legend RT Plus Centrifuge

    Swinging bucket rotor for 15 and 50 ml, and multiwell plates

  12. Sorvall RC6 Plus High Speed Centrifuge

    Rotors for volumes 1.5 - 500 ml, maximum RCF 48,000 x g

  13. Tecan M220 Plate Reader

    The Tecan M220 Plate Reader is able to take fluorescence, luminescence and absorbance readings from standard size plates with any well configurations.

  14. Tecan Spark 10M Multimode Plate Reader

    The Tecan Spark is a versatile microplate reader, capable of measuring fluorescence, absorbance, and luminescence, as well as cell confluency and cell number.